Journal: Cell Biology and Toxicology
Article Title: Andrographolide ameliorates sepsis-induced acute liver injury by attenuating endoplasmic reticulum stress through the FKBP1A-mediated NOTCH1/AK2 pathway
doi: 10.1007/s10565-025-10007-9
Figure Lengend Snippet: AP-mediated signaling ameliorates ALI by blocking ER stress. The effect of LV-NOTCH1 OE injection and TUDCA on the expression of NOTCH1 ( A ), HES1 ( B ), AK2 ( C ), CHOP ( D ), and GRP78 ( E ) in mouse liver tissues was examined using IHC staining ( n = 5). ( F ) Structural changes in liver pathology caused by LV-NOTCH1 OE injection and TUDCA detected by HE staining ( n = 5). ( G ) Effect of overexpression of NOTCH1 and blockade of ER stress pathway on apoptosis in the liver of mice detected by TUNEL ( n = 5). Data are mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ## p < 0.01, ### p < 0.001, #### p < 0.0001 by one-way ANOVA followed by Tukey’s test
Article Snippet: The ER stress inhibitor tauroursodeoxycholate (TUDCA, HY-19696, MedChemExpress, Monmouth Junction, NJ, USA) was added to drinking water at a dose of 0.5 g/kg/day (same time as AP treatment) (Qin et al. ).
Techniques: Blocking Assay, Injection, Expressing, Immunohistochemistry, Staining, Over Expression, TUNEL Assay